PWS-IC mice

Model

Jackson Laboratory (Sasner, Michael)

Bioinformatics and Model Development

The Jackson Laboratory

Michael Sasner (msasner)

Description

These PWS-ICflox6kb mice may be useful in generating conditional mutations for studying the role of Snrpn and the temporal and developmental requirements of the Prader-Willi imprinting center.

Mice homozygous for this PWS-ICflox6kb allele are viable and fertile, with loxP sites flanking the 6 kb Prader-Willi Syndrome imprinting center (PWS-IC) region around exon 1 of the Snrpn gene . When bred to mice that express Cre recombinase, the resulting offspring will have this area deleted in the cre-expressing tissue(s). Because Snrpn is imprinted and only expressed from the paternal allele, breeding PWS-ICflox6kb males with cre-expressing females is required to generate deleted offspring with the knockout phenotype. These PWS-ICflox6kb mice be useful in studying the temporal and spatial functions of the Prader-Willi imprinting center.

When crossed to female mice carrying the Tg(CMV-cre)1Cgn allele (see Stock No. 006054), widespread Cre recombinase expression prior to implantation results in postnatal lethality.

When crossed to female mice carrying the Tg(Nes-cre)1Kln allele (see Stock No.003771), Cre recombinase expression in brain precursors cells starting at E10.5 results in a growth retardation, but is not lethal.

For more information see http://jaxmice.jax.org/strain/018395.html


B6.129S1-Snrpntm2.1Kaj/J

 

DuBose AJ; Smith EY; Johnstone KA; Resnick JL. 2012. Temporal and developmental requirements for the Prader-Willi imprinting center. Proc Natl Acad Sci U S A 109(9):3446-50. [PubMed: 22331910]  [MGI Ref ID J:182162]

Dubose AJ; Smith EY; Yang TP; Johnstone KA; Resnick JL. 2011. A new deletion refines the boundaries of the murine Prader-Willi syndrome imprinting center. Hum Mol Genet 20(17):3461-6. [PubMed: 21659337]  [MGI Ref ID J:174457]

Created on: August 6, 2013 @ 1:31pm

Last Modified on: August 6, 2013 @ 1:50pm